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May 20, 2026PLoS ONEOpen Access

Estimating particle size and velocity from fluorescence pulses: A practical validation study of flow cytometry signals analysis

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Authors

MCMegan A. CattertonMDMatthew DiSalvoPPPaul N. Patrone

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Overview

Validation study shows flow cytometry can estimate particle size and velocity from fluorescence signals, highlighting accuracy in measurements.

Key Points

  • This work aims to validate how particle size, velocity, and dye concentration affect fluorescence pulse shapes in flow cytometry.
  • Controlled varying of velocity, size, and dye concentration to analyze time-resolved signals.
  • Flow rate variations were applied to microspheres with known sizes and fluorophore concentrations.
  • The method was tested on cells labeled with a DNA binding dye to assess cell cycle phases.
  • A 1.26-fold increase in the apparent diameter was observed from G0/G1 to G2 populations.
  • The volume of a sphere approximately doubled, supporting findings from literature.
  • Fluorescence pulse analysis successfully estimated particle properties, reducing uncertainty related to velocity changes.

Cite This Study

Catterton et al. (2026) studied this question.

synapsesocial.com/papers/6a0d50aef03e14405aa9c9c0https://doi.org/10.1371/journal.pone.0348292
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