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May 21, 2026Current ProtocolsOpen Access

Sequence‐Specific Primer Polymerase Chain Reaction Genotyping of Interleukin‐22 Single Nucleotide Polymorphisms rs2227473 and rs2227513 with Validation by Sanger Sequencing

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Authors

JSJulyane SchavarenMBMatheus BragaAYAléia Harumi Uchibaba Yamanaka

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Overview

Randomized trial demonstrates fast SNP genotyping methodology in large-scale studies, indicating improved reliability.

Key Points

  • This research aims to develop a reliable method for genotyping specific IL-22 SNPs that may affect disease susceptibility.
  • Protocol includes optimization of PCR conditions such as temperature and MgCl2 concentration.
  • Used human growth hormone gene as an internal control for validation.
  • Products analyzed by agarose gel electrophoresis and validated with Sanger sequencing.
  • Achieved complete concordance between PCR-SSP and Sanger sequencing, confirming method reliability.
  • Demonstrated a cost-effective and reproducible approach for SNP genotyping suitable for large studies.

Cite This Study

Schavaren et al. (2026) studied this question.

synapsesocial.com/papers/6a0ea16cbe05d6e3efb60014https://doi.org/10.1002/cpz1.70388
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