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Here, we present a protocol for establishing cutaneous squamous cell carcinoma (cSCC) in SKH-1 hairless mice using a chronic ultraviolet irradiation regimen, followed by treatment with topical 5-aminolevulinic acid photodynamic therapy (ALA-PDT). We describe steps for a 28-week UV induction schedule, standardized preparation and application of ALA cream, and precise parameters for light irradiation. The model recapitulates key stages of human photocarcinogenesis, providing a physiologically relevant system for investigating cSCC pathogenesis and assessing PDT efficacy in vivo. For complete details on the use and execution of this protocol, please refer to Chen et al.
Zhou et al. (2026) studied this question.