A dual-mode vertical flow assay for species-specific identification and total bacteria load assessment from a single urine sample

Key Points

• The aim is to develop a dual-mode assay that can identify bacteria species and assess total bacteria load from a single urine sample.
• Designed a vertical flow assay to analyze urine samples.
• Tested for both pathogen identification and quantification of bacterial load.
• Implemented species-specific detection methods.
• Successful identification of targeted pathogens in 85% of urine samples.
• Quantification of total bacteria load achieved with a sensitivity of 90%.
• Showed significant correlation between assay results and conventional laboratory methods.