Compartmentalized co-cultivation and temporal transcriptomics reveal SexM-mediated crosstalk between pheromone signaling and metabolic reprogramming in the industrial carotenoid producer Blakeslea trispora
Randomized trial reveals crosstalk between pheromone signaling and metabolic processes in Blakeslea trispora, suggesting new insights for carotenoid production.
Key Points
The research aimed to explore the interactions between pheromone signaling and metabolic changes during carotenoid production in Blakeslea trispora.
Developed a compartmentalized co-cultivation system to study pheromone signaling.
Utilized temporal transcriptomics to identify different phases of transcriptional changes.
Constructed a regulatory network focusing on the SexM factor that integrates signaling and biosynthesis processes.
Identified 887 core differentially expressed genes (DEGs) related to sexual carotenogenesis.
Revealed redox homeostasis and GPCR-MAPK signaling as key players in metabolic reprogramming due to pheromone signaling.
Established SexM as a central hub connecting pheromone signaling with carotenoid production.