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May 31, 2026The Crop Journal0 citationsOpen Access

Using male sterility for random intercrossing to increase QTL mapping resolution in self-pollinated plants

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XYXuansong Yang官官华忠ZCZhiwei Chen

Key Points

  • The study aims to enhance QTL mapping resolution in self-pollinated plants by using male sterility for creating advanced mapping populations.
  • Developed an AID-RIL population in rice using male sterility.
  • Conducted genome-wide association studies to identify quantitative-trait nucleotides.
  • Utilized the FarmCPU method for analysis.
  • Achieved high detection power for quantitative-trait nucleotides with a low false-positive rate.
  • FarmCPU demonstrated superior performance compared to other methods for identifying traits.
  • The strategy is applicable to multi-parental crosses for improved mapping.

Abstract

The purpose of this study was to develop a method for producing high-resolution mapping populations without the disadvantages of rare alleles and population structure, combining the advantages of biparental and association mapping: Advanced Intercross-Derived Recombinant Inbred Lines (AID-RILs). We used male sterility to create an AID-RIL population in rice. Genome-wide association studies in this population achieved high detection power and low false-positive rate for identifying quantitative-trait nucleotides, with the FarmCPU method showing the best performance. The strategy could be extended to multi-parental crosses

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Cite This Study

Yang et al. (2026) studied this question.

synapsesocial.com/papers/6a1bcfb05783ba022b6fbba1https://doi.org/10.1016/j.cj.2026.05.005
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