Adeno-Associated Viral Vector Purification Using a Centrifugal Microfluidic System: Towards Workflow Automation for Low-Volume Sample Processing

Key Points

• This research aims to develop an efficient method for purifying adeno-associated virus particles from crude lysate leveraging a microfluidic system.
• Developed an integrated assay for virus purification.
• Utilized a microfluidic cartridge on a centrifugal platform.
• Focused on low-volume sample processing.
• Demonstrated improved purification efficiency of AAV particles compared to traditional methods.
• Achieved significant workflow automation for low-volume samples.