The present study focuses on the formulation and evaluation of a nanostructured lipid carrier (NLC) gel incorporating Amaranthus spinosus extract for enhanced anti-inflammatory activity. Herbal extracts often face challenges such as poor stability and limited skin penetration when applied topically. To overcome these limitations, NLCs were developed as a novel drug delivery system to improve the therapeutic effectiveness of the extract. The NLCs were prepared using a combination of solid and liquid lipids along with suitable surfactants, followed by incorporation into a Carbopol-based gel. The prepared formulation was evaluated for various physicochemical parameters including particle size, polydispersity index, zeta potential, entrapment efficiency, pH, viscosity, and Spreadability. In addition, in vitro drug release, ex vivo skin permeation, and anti-inflammatory activity were assessed using standard experimental models. The optimized formulation showed nanosized particles with uniform distribution and high entrapment efficiency, indicating effective encapsulation of the extract. The gel exhibited appropriate pH and rheological properties suitable for topical application. A controlled and sustained drug release pattern was observed, along with enhanced skin permeation compared to conventional formulations. The formulation also demonstrated significant anti-inflammatory activity in both in vitro and in vivo studies. In conclusion, the developed NLC gel of Amaranthus spinosus extract offers a promising and effective approach for topical delivery, improving stability, bioavailability, and therapeutic performance. This study supports the potential of nanostructured lipid carriers as an advanced system for herbal drug delivery in the management of inflammatory conditions.
Ms. Gayatri M. Wadekar1*, Mr. Mahadev B. Mundhe2, Dr. Sampat D. Navale3 (Mon,) studied this question.