Characterisation of the Novel HLA‐C*12:459 Allele Using Third‐Generation Sequencing Methods

Key Points

• The aim is to characterize the novel HLA-C*12:459 allele and its genetic differences from HLA-C*12:02:02:01.
• Used third-generation sequencing to analyze HLA alleles.
• Identified specific nucleotide substitutions in exon 2.
• HLA-C*12:459 differs from HLA-C*12:02:02:01 by a single nucleotide substitution.