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) of twelve candidate genes, with thresholds above 200 for males and below 2 for females, wich allowed consistent separation between sexes. Among the identified transcripts, five genes (LOC102162178, ENSSSCG00000025253, USP9Y, LOC110257938, and ENSSSCG00000047835) are reported for the first time in association with sex determination in pigs. Network analysis revealed regulatory associations between these transcripts and transcription factors involved in gonadal development, including SRY, SOX9, and NOBOX. This study demonstrates that RNA-seq enables sex identification at early stages of swine development and reveals novel Y chromosome-linked transcripts in the Piau breed and their potential regulatory associations during swine sexual differentiation.
Martins et al. (Sat,) studied this question.
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