BACKGROUNDS AND AIM: Low-level laser therapy (LLLT) has been suggested to enhance cellular activity and may play a role in regenerative endodontics. This study aimed to evaluate the effect of LLLT on the adhesion of undifferentiated mesenchymal stem cells (MSCs) to human dentin. MATERIALS AND METHODS: In this ex-vivo experimental study, 63 human dentin discs were randomly allocated into three groups (n=21): irradiation with a 660 nm diode laser, irradiation with an 810 nm diode laser, and no irradiation (control). Third-passage undifferentiated MSCs were seeded onto dentin discs, and cell adhesion was assessed at 24, 48, and 72 hours using the methyl thiazolyl tetrazolium (MTT) assay. Data were analyzed using one-way ANOVA and Tukey's HSD test (α=0.05). RESULTS: LLLT significantly increased MSC adhesion compared with the control group (P<0.001). The 660 nm laser group demonstrated significantly higher cell adhesion than the 810 nm group (P<0.05). In both laser groups, cell adhesion peaked at 48 hours. CONCLUSION: LLLT enhances the adhesion of undifferentiated MSCs to human dentin, with 660 nm diode laser showing superior efficacy. These findings suggest a potential role for LLLT in regenerative endodontic and periodontal applications.
Vatanpour et al. (2026) studied this question.