• Established a liquid-extraction HILIC–LC–HRMS workflow for nasal swab metabolomics. • Compared acquired data from different nasal swab extraction volumes and confirmed 50 μL as a suitable starting sample material volume. • Demonstrated workflow feasibility via a small pilot cohort relying on matched nasal swab and plasma sample collection and analysis. • Combined (semi-)targeted metabolite quantification with non-targeted feature profiling to provide a comprehensive snapshot of nasal environment metabolomic activity.
Berger et al. (Fri,) studied this question.