New technology for monitoring NADPH metabolism in living cells

Key Points

• This research aims to develop a novel fluorescence probe to monitor NADPH metabolism in living cells and uncover its role in diseases like acute myeloid leukemia.
• Development of a series of high-performance genetically encoded fluorescent probes (iNap) for real-time monitoring.
• In situ imaging of NADPH in living cells and subcellular compartments.
• Investigation of NADPH levels in acute myeloid leukemia cells to reveal metabolic heterogeneity.
• High NADPH levels correlate with a greater abundance of functional leukemia stem cells.
• NADPH high-level subsets exhibit resistance to methotrexate treatment, mediated by MTHFD1-controlled one-carbon metabolism.