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To evaluate thymoquinone's protective effects against testicular impairment induced by boldenone undecylenate.

June 3, 2026Open Access

The modulatory role of thymoquinone in boldenone undecylenate induced testicular tissue damage: oxidative stress, inflammatory markers and androgen receptor dysregulation expression in adult male rats

Key Points

To evaluate thymoquinone's protective effects against testicular impairment induced by boldenone undecylenate.
Thirty-two male Wistar rats were divided into four groups: control, TQ 25 mg/kg orally, Bold 5 mg/kg intramuscularly, and Bold + TQ for 8 weeks.
Measured parameters included serum FSH, LH, testosterone, and testicular IL-1β, MCP-1, TNF-α, MDA, and NO levels.
Histological examination and androgen receptor expression analysis were performed on testicular tissue.
Boldenone treatment decreased serum FSH, LH, and testosterone levels while increasing oxidative stress and inflammatory markers.
Histological damage and reduced androgen receptor expression were observed in the Bold group.
Co-treatment with thymoquinone significantly mitigated these negative effects.

Abstract

OBJECTIVES: , has antioxidant and anti-inflammatory properties. To evaluate TQ's protective effects against Bold-induced testicular impairment. METHODS: Thirty-two male Wistar rats were divided into four groups (control, TQ 25 mg/kg orally, Bold 5 mg/kg intramuscularly, and Bold + TQ) for 8 weeks. Serum FSH, LH, and free testosterone, and testicular IL-1β, MCP-1, TNF-α, MDA, and NO were measured. Testes were examined histologically and for AR expression. RESULTS: Bold decreased FSH, LH, and testosterone, increased oxidative stress and inflammatory markers, caused histological damage, and reduced AR expression. TQ co-treatment significantly mitigated these effects. CONCLUSIONS: TQ protects against Bold-induced testicular injury by reducing oxidative stress and inflammation, suggesting its potential as a preventive supplement.

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