Lactiplantibacillus plantarum KLDS1.0344 ameliorates depressive-like behavior in CUMS mice: integrative multi-omics evidence linking gut microbiota alterations to sphingolipid and glycerophospholipid metabolic pathways

Key Points

• This research aims to explore how Lactiplantibacillus plantarum KLDS1.0344 affects depressive-like behavior in mice through metabolic pathways.
• Mice were subjected to chronic unpredictable mild stress (CUMS) for behavioral assessment.
• Multi-omics analysis was performed to investigate gut microbiota and metabolic pathways related to sphingolipid and glycerophospholipid metabolism.
• The effect of Lactiplantibacillus plantarum KLDS1.0344 was evaluated in comparison to control groups.
• Lactiplantibacillus plantarum KLDS1.0344 administration significantly decreased depressive-like behavior in CUMS mice, as indicated by behavioral tests.
• Alterations in gut microbiota composition linked to improvements in sphingolipid and glycerophospholipid pathways were observed, suggesting a mechanistic role in mood regulation.
• Specific metabolomic changes correlated with reduced depressive symptoms in treated mice.