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ABSTRACT This study investigated the nutritional quality of papain treated Ponmo and also monitored the biochemistry of the treatment process. Papain was extracted from different parts of pawpaw and used to treat cowhide ( Ponmo ). The results showed that the highest enzyme activity (962.10 U/ml) and protein content (3.48 mg/ml) were obtained from the pawpaw seed, while the highest specific enzyme activity (362.19 U/mg) and purification fold (1.00) were found from the leaf. Characterization studies showed the best thermal stability at 55°C with 30 min of incubation time. Casein fed animals had highest values for nutritional quality, while control recorded least values in nitrogen retention (521.29 mg/kg BW per d), biological value (46.33 %) and true digestibility (54.00 %). The lipid profile showed increase in high density lipoprotein (HDL) and decrease in low density lipoprotein (LDL). The highest values for total cholesterol (137.05 mg/dl), triglycerides (297.65 mg/dl) and HDL (90.33 mg/dl) were recorded from Ponmo treated with papain from the peels. The study has shown that when Ponmo is treated with papain, there is an increased HDL and decreased LDL, the essential amino acids become more available and quantifiable suggesting that papain treated Ponmo is more nutritive than the untreated one.
Enyi et al. (Mon,) studied this question.