This study established an ultrasonic-assisted deep eutectic solvents extraction method for the flavonoids of Crotalaria sessiliflora L. Through optimization, the optimal extraction process for the total flavonoids was determined (ChCl: LA of 1:3, water content of 16%, solid- liquid ratio of 10 mL/g, extraction time of 20 min, extraction temperature of 60 ℃, and extraction power of 200 W). Meanwhile, this study utilized the flavonoids of Crotalaria sessiliflora L. as the template molecule and employed the in situ polymerization method to synthesize multi-template flavonoid molecular imprinting polymers. Characterization, adsorption performance determination (isothermal adsorption experiments, dynamic adsorption experiments, and selectivity adsorption) were conducted, demonstrating good selectivity and adsorption performance. Additionally, multi-template molecularly imprinted polymers were used as fillers to prepare solid-phase extraction columns for optimizing the flavonoid components in the deep eutectic solvents extract, resulting in a high target recovery rate (83.16% - 86.09%). This method is low-cost, efficient, and energy-saving, providing new references and a theoretical basis for determining the content of flavonoids in Crotalaria sessiliflora L., as well as for drug research and drug safety.
Zhang et al. (Mon,) studied this question.