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June 4, 2026

The Concurrent Identification of SARS-Cov-2 and Influenza A/B Viruses in Nasopharyngeal Swabs Using Multiplex Real-time PCR.

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Authors

KHKhodaei HannanYEYari Mohammad EhsanAHArdehali Seyed Hossein

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Overview

Randomized trial investigates co-infection rates of COVID-19 and influenza A/B in hospitalized pediatric patients, suggesting implications for diagnosis and treatment.

Key Points

  • This study aims to identify SARS-CoV-2 and influenza A/B infections in pediatric patients with respiratory symptoms.
  • Collected nasopharyngeal specimens from hospitalized pediatric patients with viral respiratory infection symptoms.
  • Used multiplex real-time PCR following total RNA extraction to detect SARS-CoV-2 and influenza viruses.
  • Included 2,353 hospitalized children, with symptom data gathered via questionnaire.
  • Positive PCR results in 8% of cases: 55% for COVID-19, 8.5% for influenza A, 26% for influenza B, and 10.5% for co-infections.
  • Fever was the most commonly reported symptom among study participants.
  • Results suggest a decline in seasonal influenza incidence, potentially due to enhanced protection measures during the COVID-19 pandemic.

Cite This Study

Hannan et al. (2025) studied this question.

synapsesocial.com/papers/6a211852d499ed480b170ed3https://doi.org/10.32598/ari.80.5.3482
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Post-COVID-19 Seasonality of Influenza, Respiratory Syncytial Virus, and SARS-CoV-2 Among Hospitalized Children in Western Iran: A Molecular Surveillance Study (2023–2024)2025
  2. 2Post-COVID-19 Seasonality of Influenza, Respiratory Syncytial Virus, and SARS-CoV-2 Among Hospitalized Children in Western Iran: A Molecular Surveillance Study (2023–2024)2025 · 3 citations
  3. 3Post-COVID-19 Seasonality of Influenza, Respiratory Syncytial Virus, and SARS-CoV-2 Among Hospitalized Children in Western Iran: A Molecular Surveillance Study (2023–2024)2025 · 1 citations
  4. 4Co-infections of SARS-CoV-2 with respiratory syncytial virus and human influenza A in patients with symptoms of COVID-19 in Ghana: A retrospective study2024 · 3 citations
  5. 5Molecular Epidemiology of Respiratory Pathogens Identified by Multiplex PCR in Patients with Suspected Respiratory Tract Infection2026