The evaluation of sample preparation methods improves metabolite analysis in Atlantic salmon, suggesting actionable insights for aquaculture.
Key Points
This study aims to evaluate various sample preparation methods for NMR-based metabolomics of ovarian fluid to improve insights into reproductive success.
Tested seven sample preparation methods for NMR metabolomics analysis of ovarian fluid.
Methods included filtration, protein precipitation, lyophilization, dilution, and combinations.
Assessed the quality of metabolite spectra and the effects of blood contamination.
Filtration-concentration method yielded the highest quality spectra, with lower variability.
The method demonstrated a better signal-to-noise ratio compared to others.
Blood contamination during collection significantly affected metabolite profiles.