Disentangling Method-Induced Differences in Metabolomics: Single- versus Dual-Phase Extraction

Key Points

• The study aims to evaluate how different metabolite extraction methods affect metabolome analysis and data quality.
• Systematic comparison of single-phase and dual-phase extraction strategies.
• Assessment of metabolome coverage and data quality in both techniques.
• Focus on untargeted metabolomics applications.
• Dual-phase extraction shows improved metabolome coverage compared to single-phase extraction.
• Data quality significantly varies between the two extraction methods.
• Identifiable metabolites differ considerably based on the extraction strategy.