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April 16, 2026ChemistrySelectOpen Access

Bacterial Cellulose Synthase Catalyzes the Formation of Chitin

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Authors

NSNeraz ShaiSSSapir Korman ShadmiMPMariela J. Pavan

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Overview

Biochemical analysis reveals cellulose synthase incorporates chitin-forming substrate in bacteria, indicating enzyme versatility.

Key Points

  • To investigate whether bacterial cellulose synthase can utilize UDP-α-D-GlcNAc as a substrate for chitin production.
  • Biochemical analysis using recombinant bacterial cellulose synthase from Rhodobacter sphaeroides
  • Measurement of binding affinity and catalytic efficiency
  • Raman spectroscopic analysis of reaction products
  • Active site mutagenesis targeting specific residues
  • rBcsA-B cleaves UDP from UDP-α-D-GlcNAc with lower binding affinity and catalytic efficiency compared to UDP-α-D-Glc
  • Raman spectroscopic analysis confirms the presence of β-chitin conformation
  • Mutations in active site residues impact substrate discrimination and catalytic activity.

Cite This Study

Shai et al. (2026) studied this question.

synapsesocial.com/papers/69e07cfa2f7e8953b7cbdfd3https://doi.org/10.1002/slct.73245
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