Evaluation of ToF-SIMS imaging for semi-quantitative mapping of BODIPY-labeled fibronectin surface gradients

Key Points

• This research evaluates a new imaging method for mapping fibronectin gradients in a detailed manner.
• Developed a ToF-SIMS imaging mass spectrometry approach.
• Applied the method to microfluidic haptotaxis assays.
• Conducted comparisons with existing fluorescence microscopy techniques.
• Achieved high-resolution imaging of fibronectin gradients.
• Demonstrated improved sensitivity over traditional fluorescence methods.
• Enabled semi-quantitative analysis of surface gradients.

Abstract