Site-directed mutagenesis to enhance thermostability of Caulobacter sp. D5 ω-transaminase for efficient bioamination of biobased aldehydes

Key Points

• Enhanced thermostability allows for more efficient reactions at elevated temperatures, improving overall yield.
• Thermal stability improvements were quantified using specific mutations to the enzyme, showing significant increases in activity.
• Assessment employed site-directed mutagenesis to pinpoint critical amino acid changes responsible for increased stability at high temperatures.
• Improved enzymes facilitate broader applications in biobased chemical production, possibly influencing industrial bioprocessing methods.