Gly343 mutation enhances gentiobiose conversion efficiency of GH3 family β-glucosidase from Thermotoga maritima STB25

Key Points

• Gly343 mutation significantly increases gentiobiose conversion efficiency.
• This mutation enhances enzymatic activity by 45%, indicating substantial improvement.
• Assessment utilizes enzymatic assays to measure conversion rates in a laboratory setting.
• These findings support the potential application of modified enzymes in industrial processes.