Identifikace genů a proteinů, regulovaných Ser/Thr proteinkinasou Streptococcus pneumoniae, mikročipovou metodou a analýzou 2-D gelů

To identify the genes whose expression is controlled by protein kinase StkP we examined the transcription profile of DstkP mutant by DNA microarray technology. The transcript analysis revealed the StkP-dependent expression of many potential target loci. Out of 2,300 genes evaluated, 49 were found to be specifically and strongly upregulated in the DstkP strain, whereas 48 were downregulated. The data obtained from transcription profile were further extended by proteomic studies and mass spectrometry.