Cleanroom-Free Nanoelectrodes for Ultra-Sensitive Detection: From DNA Sensing to Electrochemical Lateral Flow Assay

The primary aim of my PhD thesis involves designing, developing, and implementing cleanroom-free nanoelectrodes. One of the main objectives is to devise a fabrication technique for producing nanoelectrodes that is both inexpensive and readily available. The study emphasizes using materials and equipment commonly found in research laboratories, making the technology more widely available and reducing the financial barrier for researchers worldwide. Another objective is to demonstrate how these nanoelectrodes can be combined into more complex devices, such as complete three-electrode systems. By integrating these nanoelectrodes into an electrochemical lateral flow assay (eLFA) system, this work seeks to enhance the sensitivity and reliability of biomarker detection for diagnostic purposes.