Significant improvement of the thermostability and catalytic efficiency of β-glucosidase Bgl59 via cysteine engineering and rational design

Key Points

• Enhanced thermostability and catalytic efficiency are achieved through cysteine engineering—transforming enzyme performance.
• The study reports a substantial increase of up to 50% in catalytic efficiency, setting a new benchmark.
• Using a rational design framework, specific cysteine residues are modified to optimize enzyme function.
• This work emphasizes the potential for engineered β-glucosidase in various bioindustrial applications.