Ratiometric naphthalimide-based fluorescent probes for highly selective detection of carboxylesterase activity

Key Points

• High selectivity for carboxylesterase activity was demonstrated in various assays, showing potential for biochemical applications.
• The detection method utilized ratiometric fluorescent probes with naphthalimide, achieving enhanced signal-to-noise ratios.
• Observational analysis assessed enzymatic activity across different substrates, highlighting variations in probe response.
• The findings support the use of these probes in diagnostic applications, enhancing specificity in biochemical assays.