Mechanistic study of italicZhenzhu Xiaoji Decoction/italic （贞术消积汤） in inhibiting liver cancer migration and invasion via the FOXO signaling pathway

Key Points

• The aim is to explore how Zhenzhu Xiaoji Decoction affects liver cancer cell migration and invasion via the FOXO signaling pathway.
• Cell scratch assay to assess migration
• Transwell assay for invasion measurements
• qRT-PCR for mRNA expression analysis
• Western blot for protein expression analysis
• In vivo study using a liver cancer xenograft mouse model
• Zhenzhu Xiaoji Decoction inhibits liver cancer cell migration and invasion in a dose-dependent manner.
• It downregulates IGF1, IGF1R, p-FOXO1, MMP2, MMP9, and VEGFA expression.
• FOXO1 and FOXO6 expression is upregulated after treatment.
• Tumor suppression rates improve and organ coefficients decline in treated groups.
• Tumor metastasis is significantly reduced after treatment.

Abstract