Abstract 2220: A spatially resolved multi-omics atlas of tertiary lymphoid structures in ovarian cancer.

Key Points

• The study aims to create a detailed atlas of tertiary lymphoid structures (TLSs) in ovarian cancer to understand their maturation and immune functions.
• Constructed a spatially resolved multi-omics atlas integrating histopathology, spatial transcriptomics, proteomics, and immunogenomics.
• Analyzed tumor samples from ovarian cancer patients using deep learning-assisted segmentation and tissue cyclical immunofluorescence (t-CyCIF).
• Performed spatial transcriptomic profiling and analyzed data for differential expression and pathway enrichment.
• Used SCORPIUS pseudotime analysis to delineate maturation trajectories of TLSs.
• Identified substantial heterogeneity in TLS and lymphoid aggregate (LAgg) abundance across different ovarian cancer histologies.
• Established distinct microstructures for immature TLSs (iTLSs) and mature TLSs (mTLSs), with mTLSs containing germinal centers.
• Profiles indicated a maturation trajectory from LAggs to iTLSs and then to mTLSs, revealing molecular insights into immune activation.

Abstract