In Silico Prioritization of High-Risk GSTO2 Missense Variants Using Consensus Pathogenicity Scoring, Static Structural Assessment, and Qualitative Docking

Glutathione S-transferase omega 2 (GSTO2) is implicated in oxidative stress management and detoxification, including reported dehydroascorbate reductase (DHAR)-related activity that supports cellular redox homeostasis. Because experimental functional data are unavailable for most GSTO2 missense variants, we performed an in silico prioritization study to rank nsSNPs for downstream validation rather than to infer causality or definitive functional effects. From 383 missense variants retrieved from dbSNP, we applied consensus pathogenicity screening (SIFT, PolyPhen-2, MutPred2, PANTHER, PhD-SNP, SNPs docking outputs were interpreted as hypothesis-generating rather than as evidence of gain- or a hypothesis for altered interaction patterns. Overall, this work delivers a ranked shortlist of GSTO2 variants with convergent computational risk signals and structure-based rationale to guide targeted biochemical and biophysical experiments.