The Effect of Ganoderma Strain Type, Growth Substrate, and Nitrogen Supplementation on The Concentration of Certain Biochemical Parameters in Fruiting Bodies

This study evaluated the efficiency of locally prepared media made from sawdust, Euphrates poplar, and crushed common reed supplemented with crushed alfalfa as a nitrogen source at 0%, 10%, 20%, and 30% levels in cultivating Chinese and local Iraqi Ganoderma lucidum. The prepared media were found to be effective in supporting the production of proteins, carbohydrates, minerals, and fatty acids (oleic acid). The Iraqi strain outperformed the Chinese strain in terms of metabolic compound production efficiency, with protein, carbohydrate, oleic acid, iron, and calcium levels of 17%, 24.548%, 2.406%, 8.11 mg kg⁻¹, and 40.55 mg kg⁻¹, respectively. The medium prepared from common reed showed higher efficiency compared to the others in cultivating both strains at 18.87%, 24.499%, 2.823%, 24.74 mg kg⁻¹, and 8.55 mg kg⁻¹, respectively for the same metabolic compounds. This shows that the protein, carbohydrate, mineral, and fatty acid content in Ganoderma lucidum varies significantly depending on the media, strain, and supplementation level