What question did this study set out to answer?

The research aims to discover small-molecule inhibitors that target the bacterial ribosome, specifically the peptidyl transferase center (PTC).

April 10, 2026Open Access

A multi-stage computational pipeline and in vitro validation for the discovery of small-molecule translation inhibitors targeting the bacterial ribosome

Key Points

The research aims to discover small-molecule inhibitors that target the bacterial ribosome, specifically the peptidyl transferase center (PTC).
Developed a hierarchical virtual screening (VS) pipeline targeting the bacterial ribosome PTC.

Structured PICO

Population

in vitro model targeting the bacterial ribosome peptidyl transferase center (PTC) of E. coli

Intervention

Mitoxantrone, Plerixafor, Olcegepant, and Ziritaxestat

Outcome

Inhibition of E. coli protein synthesis

A computational pipeline and in vitro validation successfully identified several small molecules, including Mitoxantrone, as inhibitors of bacterial protein synthesis.

Abstract

A hierarchical VS pipeline to target the bacterial ribosome PTC and in vitro translation assays identified Mitoxantrone as a potent inhibitor of E. coli protein synthesis, and Plerixafor, Olcegepant, and Ziritaxestat as lead compounds.

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Discussion

Authors

Merve Yuce

Ezgi Koman

Fethiye Aylin Sungur

Journals

RSC Advances

Actions

Institutions

Istanbul Technical University

References and Citations

Connected Papers

Building similarity graph...

Analyzing shared references across papers

A multi-stage computational pipeline and in vitro validation for the discovery of small-molecule translation inhibitors targeting the bacterial ribosome

Key Points

Structured PICO

Abstract

Citation Network

Connected Papers

Discussion

Authors

Journals

Actions

Institutions

References and Citations

Citation Network

Connected Papers

Discussion

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