CIPHER-seq enables intracellular multimodal profiling of cytokine responses in single immune cells

Key Points

• This research aims to improve the measurement of cytokines and proteins inside immune cells using a new technique called CIPHER-seq.
• Developed CIPHER-seq to quantify intracellular proteins and transcriptomes simultaneously.
• Evaluated immune cell recovery rates compared to traditional protocols.
• Applied CIPHER-seq to stimulate peripheral blood mononuclear cells (PBMCs) for analysis.
• CIPHER-seq effectively matched commercial protocols for immune cell recovery rates.
• Significantly reduced mitochondrial stress signatures were observed during analysis.
• Robust cytokine induction and metabolic changes were resolved in stimulated immune cells.

Abstract