Pilot Study to Improve In Vitro Identification of Culprit Drugs in SJS/TEN Using Granzyme B and Granulysin Secretion Assays

Key Points

• The research aims to evaluate the efficacy of GZB and granulysin detection for identifying drugs causing SJS/TEN.
• Utilized PBMC cultures to detect granzyme B and granulysin production.
• Compared assay results with conventional lymphocyte transformation tests (LTT).
• Assessed diagnostic performance at different time points, particularly day 3.
• Granzyme B detection showed higher sensitivity and specificity compared to conventional methods.
• The assay duration was significantly shorter and did not require radioactive labeling.
• Demonstrated improved diagnostic performance for drug causality in severe cutaneous adverse reactions (SCARs).

Abstract