What question did this study set out to answer?

The aim is to present a rapid protocol for genotyping Winnie mice carrying a mutation in the Muc2 gene.

April 15, 2026Open Access

Protocol for rapid allelic discrimination qPCR genotyping of the Winnie mouse model

Key Points

The aim is to present a rapid protocol for genotyping Winnie mice carrying a mutation in the Muc2 gene.
Utilized TaqMan allelic discrimination quantitative PCR for genotyping.
Conducted tissue collection and rapid crude DNA extraction.
Performed probe-based amplification using dual-labeled fluorophores.
Implemented fluorescence-based genotype calling in a single reaction.
Enabled qualitative SNP genotyping without additional processing.
Facilitated rapid analysis in a single reaction chamber.
Protocol adaptable to other defined point mutations.

Abstract

Winnie mice are a widely used in vivo model of inflammatory bowel disease and carry a missense mutation in the Muc2 gene. Here, we present a protocol for genotyping Winnie mice using TaqMan allelic discrimination quantitative PCR. We describe tissue collection, rapid crude DNA extraction, probe-based amplification with dual-labeled fluorophores, and fluorescence-based genotype calling in a single reaction. This protocol enables qualitative SNP genotyping without post-amplification processing and can be readily adapted to other defined point mutations.

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Behzad Mansoori

Chengyu Liang

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The Wistar Institute

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Protocol for rapid allelic discrimination qPCR genotyping of the Winnie mouse model

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Abstract

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Authors

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References and Citations

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