Evaluation of an In Vitro Alternative Method for Skin Irritation Using KeraSkin™

Key Points

• The study aims to evaluate the skin irritation potential of m-phenylenediamine using the KeraSkin™ model.
• Followed OECD test guideline TG 439.
• Conducted skin irritation tests with negative control (D-PBS), positive control (5% SDS), and frozen cell death controls.
• Used three skin tissues per group to ensure reproducibility.
• Quantitatively assessed cell viability using MTT analysis.
• The average cell viability in the mPDA treated group was 11.13%.
• Standard deviations from all test groups met the OECD TG 439 acceptance criteria, confirming test validity.
• Final cell viability after accounting for frozen cell death control was below 50%, classifying mPDA as a Category 2 skin irritant according to GHS standards.

Abstract