Antipyretic, Analgesic, and Anti‐Inflammatory Activities of Artocarpus chaplasha Seeds Validated by In Silico Analysis

Background The rare native Bangladeshi medicinal plant Artocarpus chaplasha has long been utilized to treat different illnesses. While its bark, leaves, and roots have folk uses, the seeds remain underexplored. The research aimed to evaluate the antipyretic, analgesic, and anti‐inflammatory properties of the methanolic seed extract to substantiate traditional medicine claims and identify therapeutic potential. Methods Mature seeds were collected, shade‐dried, powdered, and soaked in methanol. Female Swiss albino mice were utilized for in vivo assays. Antipyretic activity was assessed via pyrexia induced by brewer’s yeast, analgesic potential through acetic acid–induced writhing and paw licking tests induced by formalin, and anti‐inflammatory activity via formalin and carrageenan‐induced paw edema models. Standard drugs (paracetamol, diclofenac, morphine, and indomethacin) served as controls. Molecular docking was performed against analgesic (PDB ID: 5DU1), anti‐inflammatory (PDB ID: 5O0X), and antipyretic activity (PDB ID: 5F15) for 2 selected phytoconstituents, tetra decanoic acid, 10,13‐dimethyl‐, methyl ester, and neophytadiene. Results The extract significantly ( p < 0.005–0.001) reduced yeast‐induced hyperthermia in a dose‐dependent manner, comparable to paracetamol. Analgesic assessment showed marked writhing inhibition (56.52%) and notable decrease in paw licking duration in both neurogenic and inflammatory phases, suggesting central and peripheral antinociceptive actions. Anti‐inflammatory evaluation revealed substantial inhibition of paw edema in both models, with stronger effects at higher doses. Conclusion A. chaplasha methanolic seed extract possesses potent antipyretic, analgesic, and anti‐inflammatory activities, validating its traditional use and indicating the presence of promising bioactive compounds. Further phytochemical profiling and mechanistic studies are needed for potential plant‐derived therapeutics.