Effect of resveratrol supplementation during WooriHeukDon boar sperm cryopreservation on oxidative stress, mitochondrial function and apoptosis in porcine IVF embryos

Key Points

• The aim is to evaluate the effectiveness of resveratrol supplementation during cryopreservation in reducing oxidative stress and improving embryo development.
• Cryopreservation of boar sperm with 2 mM resveratrol during the freezing phase.
• Computer-assisted sperm analysis (CASA) used to assess motility post-thawing.
• In vitro fertilization (IVF) of embryos cultured to morula stage under uniform conditions.
• Measurement of reactive oxygen species (ROS), mitochondrial activity, and apoptotic index in embryos.
• Resveratrol supplementation significantly improved total and progressive motility of thawed sperm compared to the control group.
• Embryos from resveratrol-treated sperm showed lower ROS fluorescence intensity and higher CMXRos signal, indicating preserved mitochondrial function.
• Reduction in apoptotic index and improvements in cleavage and morula formation rates were observed in embryos derived from treated sperm.

Abstract