Visualizing Glutathione Levels in Adipose Tissue: A CBD Aryl Ether Thiolysis-Activated ESIPT Probe for Obesity Research

Key Points

• To develop a fluorescent probe for the selective detection of glutathione in adipose tissue for obesity research.
• Designed a cyanobenzoxadiazole-based fluorescent probe for glutathione detection.
• Utilized thiolysis and excited-state intramolecular proton transfer (ESIPT) for selective imaging.
• Assessed the probe's sensitivity and biocompatibility in biological samples.
• The probe showed high sensitivity for glutathione detection.
• Tunable dissociation constant (Kd) allows for specific imaging of endogenous glutathione.
• The probe was confirmed as biocompatible for use in biological tissues.

Abstract