Humanin improved the rotenone-induced reactive oxygen species formation in PC12 cells by modulating the SIRT3/Nrf2/HO-1 signaling pathway

Key Points

• The research aims to explore how humanin (HN) influences reactions in PC12 cells exposed to rotenone.
• PC12 cells were treated with rotenone and humanin.
• Expression levels of SIRT3, Nrf2, HO-1, and NQO1 proteins were measured.
• Oxidative stress indicators and the NADH ratio were evaluated.
• Humanin increased SIRT3, Nrf2, HO-1, and NQO1 expressions while reducing Ac-SOD expression.
• Activation of the Nrf2/HO-1 signaling pathway was observed in response to humanin treatment.
• Humanin effectively suppressed reactive oxygen species formation induced by rotenone.

Abstract