Effect of different irrigation regimen on sealability and push-out bond strength of biodentine when used for repair of furcal perforation in mandibular permanent molars: An in vitro study

Abstract Aim: To evaluate the influence of different irrigation regimens on the sealability and push-out bond strength of Biodentin used for furcal perforation repair in mandibular molars. Materials and Methods: Fifty-six extracted human mandibular molars with standardized 1.6 mm furcal perforations were divided into seven groups according to the irrigation protocol: (1) 1% sodium hypochlorite (NaOCl) +17% ethylenediaminetetraacetic acid (EDTA), (2) 1% NaOCl + 7% maleic acid, (3) 1% NaOCl + 9% HEDP, (4) 2% chlorhexidine (CHX) +17% EDTA, (5) 2% CHX + 7% Maleic Acid, (6) 2% CHX + 9% HEDP, and (7) saline (control). Biodentin was placed to seal the perforation and allowed to set for 24 h. Pushout bond strength was measured using a universal testing machine, and marginal sealability was analyzed under the scanning electron microscopy. Statistical analysis was performed using the one-way analysis of variance and post hoc Tukey tests. Results: Group 3 (1% NaOCl + 9% HEDP) showed the highest bond strength (31.33 ± 25.89 MPa) and the least marginal gap (1.28 ± 0.45 μm), indicating superior adhesion and sealing. Conclusion: The irrigation regimen of 1% NaOCl combined with 9% HEDP enhances Biodentin’s bonding and sealing ability in furcal perforation repair, suggesting its clinical advantage over other protocols.