Visible Spectrophotometric Estimation of Amprenavir in Pharmaceutical Formulations

Two simple, accurate, rapid and sensitive methods (A and B) have been developed for the estimation of Amprenavir in its pharmaceutical dosage form. The Method A is based on the formation of bluish-green colored chromogen, due to reaction of Amprenavir with Folin–Ciocalteu reagent (FCR) reagent in the presence of alkaline medium, which exhibits λ max at 715 nm. Method B is based on the reaction of Amprenavir with PDAC in the presence of Sulfuric acid to form a red colored chromogen, which shows maximum absorbance at 505 nm. The absorbance-concentration plot is linear over the range of 50-500 μg/mL for Method A, 10-100 μg/mL for Method B. Results of analysis for all the methods were validated statistically and by recovery studies. Literature survey reveals only HPLC methods deals with biological fluids and in fixed pharmaceutical dosage forms. The proposed methods are economical and sensitive for the estimation of Amprenavir in bulk drug and in its formulations.