Synergistic HA–tooth graft composite promotes rapid alveolar bone healing via IL-6 suppression and OCN enhancement

Periodontitis leads to continuous alveolar bone destruction driven by persistent inflammation, particularly elevated interleukin-6 (IL-6). Bone healing after tooth extraction under periodontitis conditions is often impaired, highlighting the need for regenerative biomaterials that can regulate inflammation and support osteogenesis. The hyaluronic acid–bovine tooth graft (HA + BTG) combination has been proposed as a promising BioGel-Graft candidate for accelerated bone regeneration. This study aimed to evaluate IL-6 and osteocalcin (OCN) expression as biomarkers of alveolar bone healing in Porphyromonas gingivalis –induced Wistar rats following HA + BTG administration and to compare its regenerative effects with control and HA-only groups. Thirty-six male Wistar rats were allocated into three groups (control, HA, and HA + BTG). After periodontitis induction and tooth extraction, the treatment materials were applied to the extraction sockets. Tissue samples collected on days 14 and 21 were analyzed using immunohistochemistry for IL-6 and OCN, along with hematoxylin and eosin (H BTG offers osteoconductive scaffold. • Combination biomaterial shows potential for post-extraction bone therapy.