Diagnosis of Endometriosis: Dual-Amplification Strategy Driven by Copper Nanoclusters

formation of copper nanoclusters (CuNCs), thus producing a label-free fluorescence signal within 2 h. In this design, magnetic beads not only facilitate efficient separation from serum matrices but also enhance reaction efficiency through surface-initiated enzymatic polymerization. As a result, the sensing platform exhibits excellent specificity, including reliable discrimination of single-base mismatches, and maintains robust performance in complex biological samples. This integrated platform, combining bioinformatic prescreening with a CuNCs-based sensing strategy, offers rapid, cost-effective, and label-free detection of miRNA, showing promise for early diagnosis and clinical monitoring of endometriosis.